[maker-devel] Illegal division by zero while polishing ESTs

[Carson Holt]

It’s obtaining a sequence length of zero. You may have empty entries in your sequence file. Look at the STDERR and the ID of the sequence causing the issue will be in the command name. It may be as simple as editing and removing it from your input file.

—Carson



> On Jan 22, 2020, at 1:23 AM, Ovidiu Paun <ovidiu.paun at univie.ac.at> wrote:
> 
> Hi
> I have been using maker to annotate a genome and all went fine. However I have obtained a new transcriptome (from a Trinity assembly) and implemented it as a EST fasta file. Since then I get errors for some of the contigs (but not all!):
>  
> Illegal division by zero at /usr/local/maker/bin/../lib/GI.pm line 1695.
> --> rank=NA, hostname=8767v89
> ERROR: Failed while polishig ESTs
> ERROR: Chunk failed at level:2, tier_type:3
> FAILED CONTIG:scaffold21
>  
> I checked the transcriptome fasta file and apart from the fact that it is pretty large (ca 500k contigs), I do not see any errors.
> I would greatly appreaciate any help.
> Best wishes
> Ovidiu Paun
>  
> Assoc.-Prof. Dr. Ovidiu Paun
> Department of Botany and Biodiversity Research
> University of Vienna
> http://plantgenomics.univie.ac.at <http://plantgenomics.univie.ac.at/>
>  
>  
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