[maker-devel] Request for support on maker pipeline

[Gezahegn Girma]

Hello,

I am using maker for genome annotation of sorghum genotype.

Maker is running and keep increasing the log file but I see the following error messages. This is to request for your kind support to resolve this issue.

=========================================================
		STATUS: Now running MAKER...
		WARNING: Cannot find >0, trying to re-index the fasta.

		stop here: 0

		ERROR: Fasta index error

		 at /depot/bioinfo/apps/apps/MAKER-2.31.10-nompi/bin/../lib/Process/MpiChunk.pm line 239.

		        Process::MpiChunk::_prepare(Process::MpiChunk=HASH(0x5e83458), HASH(0x5e8f9b0), 0) called at /depot/bioinfo/apps/apps/MAKER-2.31.

		10-nompi/bin/../lib/Process/MpiTiers.pm line 73

 	     	  Process::MpiTiers::__ANON__() called at /depot/bioinfo/apps/apps/MAKER-2.31.10-nompi/bin/../lib/Error.pm line 415

	     	   eval {...} called at /depot/bioinfo/apps/apps/MAKER-2.31.10-nompi/bin/../lib/Error.pm line 407

 	     	  Error::subs::try(CODE(0x5e89c60), HASH(0x5e8f6f8)) called at /depot/bioinfo/apps/apps/MAKER-2.31.10-nompi/bin/../lib/Process/MpiT

			iers.pm line 79

       		Process::MpiTiers::_prepare(Process::MpiTiers=HASH(0x5e902f8)) called at /depot/bioinfo/apps/apps/MAKER-2.31.10-nompi/bin/../lib/

		Process/MpiTiers.pm line 56

       		 Process::MpiTiers::new("Process::MpiTiers", HASH(0x5e89870), 0, "Process::MpiChunk") called at /group/bioinfo/apps/apps/MAKER-2.3

		1.10-nompi/bin/maker line 669

		--> rank=NA, hostname=bell-a105.rcac.purdue.edu <http://bell-a105.rcac.purdue.edu/>
		ERROR: Failed in tier preparation

		examining contents of the fasta file and run log

		.

		.

		.

		Warning: [blastn] Query_1 1 97 18 masked CH.. : Could not calculate ungapped Karlin-Altschul parameters due to an invalid query sequence 		or its 		

		translation. Please verify the query sequence(s) and/or filtering options

		deleted:0 hits

================================================================================

Regards,
Gez
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