From carsonhh at gmail.com Tue Nov 1 15:37:39 2022 From: carsonhh at gmail.com (Carson Holt) Date: Tue, 1 Nov 2022 15:37:39 -0600 Subject: [maker-devel] ERROR: Unknown strand in GFFDB::_sort_exons! In-Reply-To: References: Message-ID: The issue is your input GFF3 file. It has a format issue somewwhere. Likely invalid strand info or coordinate info for a feature ?Carson > On Aug 10, 2022, at 7:01 PM, Jingyin Yu wrote: > > Hello All, > > I ran maker3 and got the error message, but did not get way how to solve it, > ############################################## > ERROR: Unknown strand in GFFDB::_sort_exons! > --> rank=NA, hostname=swift > ERROR: Failed while doing repeat masking > ERROR: Chunk failed at level:0, tier_type:1 > FAILED CONTIG:chr1 > > ERROR: Chunk failed at level:2, tier_type:0 > FAILED CONTIG:chr1 > > examining contents of the fasta file and run log > ############################################## > I am not sure if you guys can give me some suggestions. Thanks a lot. > > Best, > Jingyin > > PostDoc, Boyce Thompson Institute for Plant Research? > Cornell University? > Ithaca, NY 14853? > Email: jy526 at cornell.edu ? > > _______________________________________________ > maker-devel mailing list > maker-devel at yandell-lab.org > http://yandell-lab.org/mailman/listinfo/maker-devel_yandell-lab.org -------------- next part -------------- An HTML attachment was scrubbed... URL: -------------- next part -------------- A non-text attachment was scrubbed... Name: smime.p7s Type: application/pkcs7-signature Size: 1376 bytes Desc: not available URL: From carsonhh at gmail.com Thu Nov 3 11:47:26 2022 From: carsonhh at gmail.com (Carson Holt) Date: Thu, 3 Nov 2022 11:47:26 -0600 Subject: [maker-devel] Maker always fails to run on a particular chromosome. In-Reply-To: References: Message-ID: <34134473-FDD6-4030-829B-DE3D7B532761@gmail.com> You have a number of initial GFF3 related errors. I think one of your input files has an issue somewhere. Likely a truncated line in the GFF3. You will have to find and fix the feature with issues, then delete any *.db files under the *.maker.output directory before restarting. That will force it to rebuild the database that maps the current GFF3 files. Thanks, Carson > On Oct 23, 2022, at 12:08 AM, WIIE AY wrote: > > Hi, > > Thanks for this excellent software. > However, when we annotated a rice genome (chr1-12) using Maker/3.01.03, the first round ran very well. > When we added the snap and gmhmm models in the second round, only chr04 always failed in 12 chromosomes. > Here is my chr4_run.log, stderr.out.(Maybe need maker_opts.ctl.) > Looking forward to your reply. > > ?maker_opts.ctl > ?run.log > ?stderr_73012967.err THANKS > Peter > _______________________________________________ > maker-devel mailing list > maker-devel at yandell-lab.org > http://yandell-lab.org/mailman/listinfo/maker-devel_yandell-lab.org -------------- next part -------------- An HTML attachment was scrubbed... URL: -------------- next part -------------- A non-text attachment was scrubbed... Name: smime.p7s Type: application/pkcs7-signature Size: 1376 bytes Desc: not available URL: From elissaralam at ufl.edu Wed Nov 23 07:46:08 2022 From: elissaralam at ufl.edu (Alam,Elissar) Date: Wed, 23 Nov 2022 14:46:08 +0000 Subject: [maker-devel] Augustus predictions within MAKER2 Message-ID: Hello, I ran Augustus and SNAP as part of the MAKER2 gene annotation pipeline. I would like to use the gene predictions generated by Augustus (prior to MAKER selecting the final gene model based on all ab initio predictions) for downstream analyses. Is there a way to obtain the Augustus gene predictions in gff3 format from MAKER2? Thanks a million, Elissar -------------- next part -------------- An HTML attachment was scrubbed... URL: From jason.stajich at gmail.com Fri Nov 25 09:49:55 2022 From: jason.stajich at gmail.com (Jason Stajich) Date: Fri, 25 Nov 2022 08:49:55 -0800 Subject: [maker-devel] Augustus predictions within MAKER2 In-Reply-To: References: Message-ID: After you run the gff3_merge step you can grep augustus final.gff > augustus.gff3 On Wed, Nov 23, 2022 at 8:02 AM Alam,Elissar wrote: > Hello, > > > > I ran Augustus and SNAP as part of the MAKER2 gene annotation pipeline. I > would like to use the gene predictions generated by Augustus (prior to > MAKER selecting the final gene model based on all ab initio predictions) > for downstream analyses. Is there a way to obtain the Augustus gene > predictions in gff3 format from MAKER2? > > > > Thanks a million, > > Elissar > _______________________________________________ > maker-devel mailing list > maker-devel at yandell-lab.org > http://yandell-lab.org/mailman/listinfo/maker-devel_yandell-lab.org > -- Jason Stajich jason.stajich at gmail.com -------------- next part -------------- An HTML attachment was scrubbed... URL: From elissaralam at ufl.edu Fri Nov 25 09:56:49 2022 From: elissaralam at ufl.edu (Alam,Elissar) Date: Fri, 25 Nov 2022 16:56:49 +0000 Subject: [maker-devel] Augustus predictions within MAKER2 In-Reply-To: References: Message-ID: Would that include all the gene models predicted by Augustus or only the ones that Maker retains after considering all ab initio gene predictions (SNAP + Augustus) and the EST evidence? Thanks a lot, Elissar Sent from Mail for Windows From: Jason Stajich Sent: Friday, November 25, 2022 11:51 AM To: Alam,Elissar Cc: maker-devel at yandell-lab.org Subject: Re: [maker-devel] Augustus predictions within MAKER2 [External Email] After you run the gff3_merge step you can grep augustus final.gff > augustus.gff3 On Wed, Nov 23, 2022 at 8:02 AM Alam,Elissar > wrote: Hello, I ran Augustus and SNAP as part of the MAKER2 gene annotation pipeline. I would like to use the gene predictions generated by Augustus (prior to MAKER selecting the final gene model based on all ab initio predictions) for downstream analyses. Is there a way to obtain the Augustus gene predictions in gff3 format from MAKER2? Thanks a million, Elissar _______________________________________________ maker-devel mailing list maker-devel at yandell-lab.org http://yandell-lab.org/mailman/listinfo/maker-devel_yandell-lab.org -- Jason Stajich jason.stajich at gmail.com -------------- next part -------------- An HTML attachment was scrubbed... URL: From jason.stajich at gmail.com Sat Nov 26 12:29:27 2022 From: jason.stajich at gmail.com (Jason Stajich) Date: Sat, 26 Nov 2022 11:29:27 -0800 Subject: [maker-devel] Augustus predictions within MAKER2 In-Reply-To: References: Message-ID: I?d check the documentation and the raw files in the run folder if you aren?t sure but The maker consensus models will have maker in the 2nd column and the augustus will have augustus in second column of gff (?source?) You might need to adjust your grep to only take those with augustus in 2nd Col. you can do grep -P ?\taugustus\t? to get those. On Fri, Nov 25, 2022 at 8:56 AM Alam,Elissa wrote: > Would that include all the gene models predicted by Augustus or only the > ones that Maker retains after considering all ab initio gene predictions > (SNAP + Augustus) and the EST evidence? > > > > Thanks a lot, > > Elissar > > > > Sent from Mail for > Windows > > > > *From: *Jason Stajich > *Sent: *Friday, November 25, 2022 11:51 AM > *To: *Alam,Elissar > *Cc: *maker-devel at yandell-lab.org > *Subject: *Re: [maker-devel] Augustus predictions within MAKER2 > > > > *[External Email]* > > After you run the gff3_merge step you can > > grep augustus final.gff > augustus.gff3 > > > > On Wed, Nov 23, 2022 at 8:02 AM Alam,Elissar wrote: > > Hello, > > > > I ran Augustus and SNAP as part of the MAKER2 gene annotation pipeline. I > would like to use the gene predictions generated by Augustus (prior to > MAKER selecting the final gene model based on all ab initio predictions) > for downstream analyses. Is there a way to obtain the Augustus gene > predictions in gff3 format from MAKER2? > > > > Thanks a million, > > Elissar > > _______________________________________________ > maker-devel mailing list > maker-devel at yandell-lab.org > http://yandell-lab.org/mailman/listinfo/maker-devel_yandell-lab.org > > > -- > > Jason Stajich > jason.stajich at gmail.com > > > -- Jason Stajich jason.stajich at gmail.com -------------- next part -------------- An HTML attachment was scrubbed... URL: From carsonhh at gmail.com Tue Nov 1 15:37:39 2022 From: carsonhh at gmail.com (Carson Holt) Date: Tue, 1 Nov 2022 15:37:39 -0600 Subject: [maker-devel] ERROR: Unknown strand in GFFDB::_sort_exons! In-Reply-To: References: Message-ID: The issue is your input GFF3 file. It has a format issue somewwhere. Likely invalid strand info or coordinate info for a feature ?Carson > On Aug 10, 2022, at 7:01 PM, Jingyin Yu wrote: > > Hello All, > > I ran maker3 and got the error message, but did not get way how to solve it, > ############################################## > ERROR: Unknown strand in GFFDB::_sort_exons! > --> rank=NA, hostname=swift > ERROR: Failed while doing repeat masking > ERROR: Chunk failed at level:0, tier_type:1 > FAILED CONTIG:chr1 > > ERROR: Chunk failed at level:2, tier_type:0 > FAILED CONTIG:chr1 > > examining contents of the fasta file and run log > ############################################## > I am not sure if you guys can give me some suggestions. Thanks a lot. > > Best, > Jingyin > > PostDoc, Boyce Thompson Institute for Plant Research? > Cornell University? > Ithaca, NY 14853? > Email: jy526 at cornell.edu ? > > _______________________________________________ > maker-devel mailing list > maker-devel at yandell-lab.org > http://yandell-lab.org/mailman/listinfo/maker-devel_yandell-lab.org -------------- next part -------------- An HTML attachment was scrubbed... URL: -------------- next part -------------- A non-text attachment was scrubbed... Name: smime.p7s Type: application/pkcs7-signature Size: 1376 bytes Desc: not available URL: From carsonhh at gmail.com Thu Nov 3 11:47:26 2022 From: carsonhh at gmail.com (Carson Holt) Date: Thu, 3 Nov 2022 11:47:26 -0600 Subject: [maker-devel] Maker always fails to run on a particular chromosome. In-Reply-To: References: Message-ID: <34134473-FDD6-4030-829B-DE3D7B532761@gmail.com> You have a number of initial GFF3 related errors. I think one of your input files has an issue somewhere. Likely a truncated line in the GFF3. You will have to find and fix the feature with issues, then delete any *.db files under the *.maker.output directory before restarting. That will force it to rebuild the database that maps the current GFF3 files. Thanks, Carson > On Oct 23, 2022, at 12:08 AM, WIIE AY wrote: > > Hi, > > Thanks for this excellent software. > However, when we annotated a rice genome (chr1-12) using Maker/3.01.03, the first round ran very well. > When we added the snap and gmhmm models in the second round, only chr04 always failed in 12 chromosomes. > Here is my chr4_run.log, stderr.out.(Maybe need maker_opts.ctl.) > Looking forward to your reply. > > ?maker_opts.ctl > ?run.log > ?stderr_73012967.err THANKS > Peter > _______________________________________________ > maker-devel mailing list > maker-devel at yandell-lab.org > http://yandell-lab.org/mailman/listinfo/maker-devel_yandell-lab.org -------------- next part -------------- An HTML attachment was scrubbed... URL: -------------- next part -------------- A non-text attachment was scrubbed... Name: smime.p7s Type: application/pkcs7-signature Size: 1376 bytes Desc: not available URL: From elissaralam at ufl.edu Wed Nov 23 07:46:08 2022 From: elissaralam at ufl.edu (Alam,Elissar) Date: Wed, 23 Nov 2022 14:46:08 +0000 Subject: [maker-devel] Augustus predictions within MAKER2 Message-ID: Hello, I ran Augustus and SNAP as part of the MAKER2 gene annotation pipeline. I would like to use the gene predictions generated by Augustus (prior to MAKER selecting the final gene model based on all ab initio predictions) for downstream analyses. Is there a way to obtain the Augustus gene predictions in gff3 format from MAKER2? Thanks a million, Elissar -------------- next part -------------- An HTML attachment was scrubbed... URL: From jason.stajich at gmail.com Fri Nov 25 09:49:55 2022 From: jason.stajich at gmail.com (Jason Stajich) Date: Fri, 25 Nov 2022 08:49:55 -0800 Subject: [maker-devel] Augustus predictions within MAKER2 In-Reply-To: References: Message-ID: After you run the gff3_merge step you can grep augustus final.gff > augustus.gff3 On Wed, Nov 23, 2022 at 8:02 AM Alam,Elissar wrote: > Hello, > > > > I ran Augustus and SNAP as part of the MAKER2 gene annotation pipeline. I > would like to use the gene predictions generated by Augustus (prior to > MAKER selecting the final gene model based on all ab initio predictions) > for downstream analyses. Is there a way to obtain the Augustus gene > predictions in gff3 format from MAKER2? > > > > Thanks a million, > > Elissar > _______________________________________________ > maker-devel mailing list > maker-devel at yandell-lab.org > http://yandell-lab.org/mailman/listinfo/maker-devel_yandell-lab.org > -- Jason Stajich jason.stajich at gmail.com -------------- next part -------------- An HTML attachment was scrubbed... URL: From elissaralam at ufl.edu Fri Nov 25 09:56:49 2022 From: elissaralam at ufl.edu (Alam,Elissar) Date: Fri, 25 Nov 2022 16:56:49 +0000 Subject: [maker-devel] Augustus predictions within MAKER2 In-Reply-To: References: Message-ID: Would that include all the gene models predicted by Augustus or only the ones that Maker retains after considering all ab initio gene predictions (SNAP + Augustus) and the EST evidence? Thanks a lot, Elissar Sent from Mail for Windows From: Jason Stajich Sent: Friday, November 25, 2022 11:51 AM To: Alam,Elissar Cc: maker-devel at yandell-lab.org Subject: Re: [maker-devel] Augustus predictions within MAKER2 [External Email] After you run the gff3_merge step you can grep augustus final.gff > augustus.gff3 On Wed, Nov 23, 2022 at 8:02 AM Alam,Elissar > wrote: Hello, I ran Augustus and SNAP as part of the MAKER2 gene annotation pipeline. I would like to use the gene predictions generated by Augustus (prior to MAKER selecting the final gene model based on all ab initio predictions) for downstream analyses. Is there a way to obtain the Augustus gene predictions in gff3 format from MAKER2? Thanks a million, Elissar _______________________________________________ maker-devel mailing list maker-devel at yandell-lab.org http://yandell-lab.org/mailman/listinfo/maker-devel_yandell-lab.org -- Jason Stajich jason.stajich at gmail.com -------------- next part -------------- An HTML attachment was scrubbed... URL: From jason.stajich at gmail.com Sat Nov 26 12:29:27 2022 From: jason.stajich at gmail.com (Jason Stajich) Date: Sat, 26 Nov 2022 11:29:27 -0800 Subject: [maker-devel] Augustus predictions within MAKER2 In-Reply-To: References: Message-ID: I?d check the documentation and the raw files in the run folder if you aren?t sure but The maker consensus models will have maker in the 2nd column and the augustus will have augustus in second column of gff (?source?) You might need to adjust your grep to only take those with augustus in 2nd Col. you can do grep -P ?\taugustus\t? to get those. On Fri, Nov 25, 2022 at 8:56 AM Alam,Elissa wrote: > Would that include all the gene models predicted by Augustus or only the > ones that Maker retains after considering all ab initio gene predictions > (SNAP + Augustus) and the EST evidence? > > > > Thanks a lot, > > Elissar > > > > Sent from Mail for > Windows > > > > *From: *Jason Stajich > *Sent: *Friday, November 25, 2022 11:51 AM > *To: *Alam,Elissar > *Cc: *maker-devel at yandell-lab.org > *Subject: *Re: [maker-devel] Augustus predictions within MAKER2 > > > > *[External Email]* > > After you run the gff3_merge step you can > > grep augustus final.gff > augustus.gff3 > > > > On Wed, Nov 23, 2022 at 8:02 AM Alam,Elissar wrote: > > Hello, > > > > I ran Augustus and SNAP as part of the MAKER2 gene annotation pipeline. I > would like to use the gene predictions generated by Augustus (prior to > MAKER selecting the final gene model based on all ab initio predictions) > for downstream analyses. Is there a way to obtain the Augustus gene > predictions in gff3 format from MAKER2? > > > > Thanks a million, > > Elissar > > _______________________________________________ > maker-devel mailing list > maker-devel at yandell-lab.org > http://yandell-lab.org/mailman/listinfo/maker-devel_yandell-lab.org > > > -- > > Jason Stajich > jason.stajich at gmail.com > > > -- Jason Stajich jason.stajich at gmail.com -------------- next part -------------- An HTML attachment was scrubbed... URL: