[maker-devel] query

indu khatri indu2287 at gmail.com
Wed Dec 19 22:33:33 MST 2012


 Hello Maker-devel
    Thanks for adding me to the list. I am new to this field. I have a
query regarding maker annotation.
    I have assembled a fungal Genome with 1590 contigs and was trying to
    annotate it using MAKER pipeline. I only have paired end WGS sequence
from
    Illumina HiSeq sequencing which were assembled using CLCbio. I dont have
    any transcriptome data. I am using ESTs of a closer species for
validation
    and similarly the protein sequences. I have started annotation but the
    problem is the pipeline gets stuck to the first contig on blastx step
and
    doesn't move further. I could not make out the problem. Can you please
help
    me out of this.

Thanks and regards

-- 
<https://mail.google.com/mail/?ui=2&ik=8ec2492227&view=att&th=130da06dcd7f8e6a&attid=0.0.1&disp=emb&realattid=b8967bbd6e93d49d_0.25&zw>Indu
Khatri
PhD Research Fellow,
C/o Dr. Srikrishna Subramanian
Institute of Microbial Technology,
Chandigarh-160036,
India
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