[maker-devel] maker-2.23 with tophat / cufflinks input

Olaf Mueller olaf.mueller at duke.edu
Thu Feb 23 10:16:58 MST 2012


Hi,

I've done a preliminary run on an alga genome with maker 2.23beta, using 
only mRNA-seq tophat/cufflinks input and no additional evidence source.

As prerequisite a cufflinks transcripts.gtf (merged from different read 
sample mappings) has been converted with maker cufflinks2gff3 
(strandless transcripts/exons removed). I provided the resulting gff3 
via pred_gff and model_gff to maker, and also didn't call est2genome or 
other predictors.

During maker run for some scaffolds, I receive this error message for 
some chunks, while gathering GFF3 input into hits:

Gathering GFF3 input into hits - chunk:0
panic: fold_constants JMPENV_PUSH returned 2 at 
/home/muol/Documents/Software/maker/lib/File/..//Proc/Signal.pm line 89
Gathering GFF3 input into hits - chunk:2
...

The index log nevertheless reports these scaffolds as finished and 
annotations are generated. Do I have to be concerned about this message 
because it is related to my cufflinks input?

Thanks
Olaf





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