[maker-devel] Strange error at blastn step

[Daniel Standage]

Greetings!

I am doing a test run of my Maker setup on a new machine, annotating a
pretty short contig (about 3kb). However, there seems to be a hiccup during
the blastn stage. This is the terminal message.

#--------- command -------------#
Widget::blastn:
/share/home/01854/standage/local/bin/blastn -db
/tmp/2881473.1.development/maker_i6ZSJi/Pdom%2ETrinity%2ETrimmomatic%2Efasta.mpi.10.7
-query /tmp/2881473.1.development/maker_i6ZSJi/rank0/scaffold_866.0
-num_alignments 100000 -num_descriptions 100000 -evalue 1e-10 -gapextend 3
-word_size 15 -reward 1 -penalty -3 -gapopen 3 -dbsize 1000 -searchsp
500000000 -num_threads 16 -lcase_masking -dust yes -soft_masking true -show_gis
-out
/scratch/01854/standage/PdomGenomic/Annotation/scripts/maker.bogus.maker.output/maker.bogus_datastore/scaffold_866/theVoid.scaff
old_866/scaffold_866.0.Pdom%2ETrinity%2ETrimmomatic%2Efasta.blastn.temp_dir/Pdom%2ETrinity%2ETrimmomatic%2Efasta.mpi.10.7.blastn
#-------------------------------#
deleted:0 hits
ERROR:  Could not obtain lock to format database


FATAL ERROR
ERROR: Failed while doing blastn of ESTs!!

ERROR: Chunk failed at level 8
!!
FAILED CONTIG:scaffold_866


Several blastn steps appeared to have completed successfully to this one
failing. Any ideas what could be causing this?

Thanks!

--
Daniel S. Standage
Ph.D. Candidate
Bioinformatics and Computational Biology Program
Department of Genetics, Development, and Cell Biology
Iowa State University
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