[maker-devel] Annotation with maker2

Chris Bioinfo chrisbioinfo at gmail.com
Wed Mar 19 05:09:57 MDT 2014


Hello,

I'm installing/using maker2 for the first time and I have an error by using
it.

I certainly missing something, but I don't know what.

I compile maker with no error message and I have all these directories
after compilation:
bin  data  GMOD  INSTALL  lib  LICENSE  MWAS  perl  README  src

Nevertheless when I try maker2 on the test data (dpp_contig.fasta) I have
this error:

STATUS: Now running MAKER...
examining contents of the fasta file and run log



--Next Contig--

#---------------------------------------------------------------------
Now starting the contig!!
SeqID: contig-dpp-500-500
Length: 32156
#---------------------------------------------------------------------


setting up GFF3 output and fasta chunks
doing repeat masking
DBI connect('dbname=/path/to/dpp_contig.maker.output/dpp_contig.db','',...)
failed: unable to open database file at
/usr/local/annotation/maker2.31/bin/../lib/GFFDB.pm

Can't call method "do" on an undefined value at
/usr/local/annotation/maker2.31/bin/../lib/GFFDB.pm
--> rank=NA, hostname=belem
ERROR: Failed while doing repeat masking
ERROR: Chunk failed at level:0, tier_type:1
FAILED CONTIG:contig-dpp-500-500
...

ideas?

Best,

Christelle
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