[maker-devel] MAKER contig: Non-unique top level ID

Daniel Ence dence at genetics.utah.edu
Fri Oct 24 07:51:25 MDT 2014


Hi Irina,

Have you looked through your input for the duplicate IDs that the error talks about? It looks like some of your input files contain features with the same ID.

~Daniel


On Oct 24, 2014, at 7:17 AM, Irina Armean <iarmean at ebi.ac.uk<mailto:iarmean at ebi.ac.uk>> wrote:

Dear all,

I can't get a subset of contigs to finish, they keep failing.

The log below is of a 519 992 long and max_dna_len=100000. No gff file is created. Just evidence_0.gff.an<http://evidence_0.gff.an/> contains data (blast, protein2genome), the other 4 are empty (evidence_1, evidence_2, evidence_3, evidence_4).


...processing 30 of 32
 ...processing 31 of 32
total clusters:5 now processing 0
flattening protein clusters
prepare section files
Gathering GFF3 input into hits - chunk:0
ERROR: Non-unique top level ID for
While this is technically legal in GFF3, it usually
indicates a poorly fomatted GFF3 file (perhaps you
tried to merge two GFF3 files without accounting for
unique IDs).  MAKER will not handle these correctly.

--> rank=NA, hostname=ebi3-202.ebi.ac.uk<http://ebi3-202.ebi.ac.uk/>
ERROR: Failed while prepare section files
ERROR: Chunk failed at level:12, tier_type:3
FAILED CONTIG:scaffold2

ERROR: Chunk failed at level:4, tier_type:0
FAILED CONTIG:scaffold2

examining contents of the fasta file and run log



--Next Contig--

Any suggestions much appreciated.

Best wishes,
Irina

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