[maker-devel] MAKER contig: Non-unique top level ID
Irina Armean
iarmean at ebi.ac.uk
Fri Oct 24 07:17:51 MDT 2014
Dear all,
I can't get a subset of contigs to finish, they keep failing.
The log below is of a 519 992 long and max_dna_len=100000. No gff file is
created. Just evidence_0.gff.an contains data (blast, protein2genome), the
other 4 are empty (evidence_1, evidence_2, evidence_3, evidence_4).
...processing 30 of 32
...processing 31 of 32
total clusters:5 now processing 0
flattening protein clusters
prepare section files
Gathering GFF3 input into hits - chunk:0
ERROR: Non-unique top level ID for
While this is technically legal in GFF3, it usually
indicates a poorly fomatted GFF3 file (perhaps you
tried to merge two GFF3 files without accounting for
unique IDs). MAKER will not handle these correctly.
--> rank=NA, hostname=ebi3-202.ebi.ac.uk
ERROR: Failed while prepare section files
ERROR: Chunk failed at level:12, tier_type:3
FAILED CONTIG:scaffold2
ERROR: Chunk failed at level:4, tier_type:0
FAILED CONTIG:scaffold2
examining contents of the fasta file and run log
--Next Contig--
Any suggestions much appreciated.
Best wishes,
Irina
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