[maker-devel] problem with the example

Alejandro Cerón Noriega alejocn5 at gmail.com
Tue Feb 16 14:17:40 MST 2016


hello i am Alejandro

I have tried to follow the tutorial MAKER

1-I Copy the files in the data directories to a temporary directory
where i  run an example file.
2-I Type maker -CTL to generate generic MAKER control files (foto_1)
3-I  edit the control files to include the path of    the genome file  (
hsap_contig.fasta from the example) (foto_2)
then I  give the paht maker maker_exe.ctl maker_opts.ctl maker_bopts.ctl
(foto 3)

that generate a expected folder
hsap_contig.maker.output

but when i whatn to look for the gff file i dont find it, inside the
/data/hsap_contig.maker.output/hsap_contig_datastore, i dont find  the all
subdirectories

seq_name.gff - a gff file that can be loaded into GMOD, GBROWSE,
      or Apollo
    * seq_name.maker.transcripts.fasta - a fasta file of the MAKER
      annotated transcript sequences
    * seq_name.maker.proteins.fasta - a fasta file of the MAKER
      annotated protein sequences
    * seq_name.maker.XXX.transcript.fasta - a fasta file of ab-initio
      predicted transcript sequences from program XXX
    * seq_name.maker.XXX.proteins.fasta - a fasta file of ab-inito
      predicted protein sequences from program XXX
    * seq_name.maker.non_overlapping_ab_initio.transcripts.fasta - a
      fasta file of filtered ab-inito transcript sequences that don't
      overlap maker annotations
    * seq_name.maker.non_overlapping_ab_initio.proteins.fasta - a
      fasta file of filtered ab-inito protein sequences that don't
      overlap maker annotations
    * theVoid.seq_name/ - a directory containing all of the raw
      output files produced by MAKER, including BLAST reports, SNAP
      output, exonnerate output and the masked genomeic sequence.

i only find a directorie named 80 (foto 4)

i dont know if a make somthing wrong,

also try to change the path of the EST (foto_5)


thanks for your attention


-- 
*Alejandro Cerón Noriega, **B.Sc*
MSc. Candidate Bioinformatics
*K ♣**♣**♣*
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