[maker-devel] ProtExcluder1.2 Error

[Prashant S Hosmani]

Hi Amine,

I was getting similar error. You need to be careful with the blast versions. Try using the same blast version for makeblastdb. I was using BLAST 2.2.29+. After recreating new blast database with same version, it worked for me.

Hope this helps.
Prashant


Prashant Hosmani
Sol Genomics Network
Boyce Thompson Institute, Ithaca, NY, USA



On Nov 3, 2016, at 1:57 PM, Michael Campbell <michael.s.campbell1 at gmail.com<mailto:michael.s.campbell1 at gmail.com>> wrote:

Hi Amine,

That script is maintained by Ning Jiang and Kevin Childs. They know best what this script is expecting. I’ve ccd them on this email in the hope that they can provide some direction.

Thanks,
Mike
On Nov 2, 2016, at 2:09 PM, Mohamed Amine Chebbi <mohamed.amine.chebbi at univ-poitiers.fr<mailto:mohamed.amine.chebbi at univ-poitiers.fr>> wrote:

Hi!

I am working on creating a custom repeat library and I want to use ProtExcluder1.2 to trim potential genes from my repeat sequences.
My blast version is BLAST 2.2.30+

I get this message error :

Can not open the seqfile test.lib_blast_results.txt.fnolowm50seq
mergeunmatchedregion.pl seqfile
Illegal division by zero at ProtExcluder1.2/GCcontent.pl line 122.

I wonder if you can help me to fix this.

Thank you.

Amine
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