[maker-devel] altEST evidence
Miche Zacharias
miche.zacharias at gmail.com
Tue Apr 25 05:35:37 MDT 2017
Hi,
Would there be any benefit to passing CDS instead of cDNA sequences as
alternative EST evidence, if both is available? Or when would you use one
or the other?
I've read in previous discussions how it's expected that the organism, from
which we use sequences as alternative EST and protein evidence, is not
closely related to the genome we are annotating.
In what terms is "closely related" defined? It's now unclear to me when you
would put EST/protein evidence as alternative and when not or put it as
direct EST/protein evidence?
Would a species from the same family classify as alternative or direct
evidence? Is ~30mya divergence too much?
What about species of the same genus?
Many thanks!
Miche
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