[maker-devel] Error trying to submit genome to ncbi

[Emmanuel Nnadi]

Hi,

I am trying to submit my genome i annotated using maker and they sent back
this error,
1. Please remove any N nucleotides from the beginning or end of the sequence
 2.No feature should begin or end inside a gap.  Instead the feature should
be made partial at the gap boundary.

[3] Coding regions should not be 5' partial if they begin with the start
methionine.  If this is an internal methionine int he translation than
it is fine if they are partial.  Conversely, all coding regions
must have a stop codon or be 3' partial.
You have a large number of gene features that are not associated
with other features.  Please include on these features in the
gene description field some description of what the gene would
have encoded.

A feature table example of this is:

<41156  >40652  gene
                        gene_desc       transposon
                        locus_tag       CR513_45338
                        note    nonfunctional due to frameshift
Please how can i use maker to solve this problem?


Nnadi Nnaemeka Emmanuel
Department of Microbiology,
Faculty of Natural and Applied Science,
Plateau State University, Bokkos, Plateau State, Nigeria.
Publications:
https://www.researchgate.net/profile/Emmanuel_Nnadi/publications
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