[maker-devel] NNN in maker output transcript

Daniel Ence dandence at gmail.com
Wed Oct 25 09:42:04 MDT 2017


Hi Wen Yao, 

Do you mean that some of the transcript sequences contain “N” characters or that an entire transcript sequence is “NNN”?  



> On Oct 25, 2017, at 3:25 AM, Wen Yao <venyao at qq.com> wrote:
> 
> Dear guys,
> 
> Recently, I run maker to annotate a genome. I found that the transcript fasta file output by Maker contains "NNN". Is this normal?
> If not, what's going on? Is this a bug of maker or my configuration of maker is not correct? 
> I told maker to use snap and augustus for de novo prediction and use exonerate to align ESTs and proteins.
> 
> Thanks!
> 
> Wen Yao
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