[maker-devel] Annotations from high confidence IsoSeq data

[Carson Holt]

MAKER doesn’t assign weights to evidence types, but you can look into EVAL which can take MAKER results and produce new models. If you gave your evidence as est= then EVAL can consume that as well since it will be in the GFF3. MAKER3-beta have some integrated support for eval that you can look at using the maker_eval.ctl file to provide weights to EVAL.

—Carson


> On Jul 10, 2019, at 12:12 PM, DECKER, KEITH F [AG/1005] <keith.decker at bayer.com> wrote:
> 
> I have a set of high confidence full length transcripts generated via the pacbio IsoSeq pipeline. 
>  
> Is there a recommended way to pass these transcripts to MAKER and ensure that the corresponding gene models are included in the final annotation?  I also have assembled illumina transcripts, but I’m trying to figure out how to give the IsoSeq transcripts a higher ‘weight’.
>  
> 
> 
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