[maker-devel] Contamination report from NCBI

[Xabier Vázquez-Campos]

Hi there,
Did you perform quality and adapter trimming of your raw reads? That's
actually an assembly issue.
I would seriously encourage you to redo the assembly before continuing.

If that isnt possible, start by removing those sequences and split the
contigs at those places as suggested in the report.

For the annotation part, not 100% sure but I'd say start with the
"Merge/resolve legacy annotations" steps but maybe Carson or Daniel have a
different suggestion
http://weatherby.genetics.utah.edu/MAKER/wiki/index.php/MAKER_Tutorial_for_GMOD_Online_Training_2014#Merge.2FResolve_Legacy_Annotations

Cheers,
Xabi


On 24 October 2017 at 00:30, Emmanuel Nnadi <eennadi at gmail.com> wrote:

> Hello
>
> Good day.
>
> Please I submitted my sequence to NCBI and they sent back this
> contamination report.
>
> Please how do I use maker to effect the correction
>
>
> Nnadi Nnaemeka Emmanuel
> Department of Microbiology,
> Faculty of Natural and Applied Science,
> Plateau State University, Bokkos, Plateau State, Nigeria.
> Publications:  https://www.researchgate.net/profile/Emmanuel_Nnadi/
> publications
>
> _______________________________________________
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>
>


-- 
Xabier Vázquez-Campos, *PhD*
*Research Associate*
NSW Systems Biology Initiative
School of Biotechnology and Biomolecular Sciences
The University of New South Wales
Sydney NSW 2052 AUSTRALIA
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